Monovalent cation composition and ATP and lipid content of irreversibly sickled cells
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چکیده
منابع مشابه
Monovalent cation composition and ATP and lipid content of irreversibly sickled cells.
Discontinuous Stractan gradients were used to separate heterogeneous populations of sickle cells into discrete subpopulations containing varying proportions of reticulocytes, mature discoid cells, and irreversibly sickled cells (ISC). The improved homogeneity of these preparations, together with an enhanced yield of ISC, allowed us to distinguish effects of cell maturation from those of irrever...
متن کاملMonovalent cation transport in irreversibly sickled cells.
Using discontinuous density gradients of Stractan II, we have separated sickle cell blood into discrete subpopulations of reticulocytes, mature discoid cells, and irreversibly sickled cells (ISCs). We have measured active and passive fluxes of monovalent cations in mature discoid cells, ISCs, and normal control cells, also separated upon density gradients. These measurements revealed a decrease...
متن کاملCation permeability alterations during sickling: relationship to cation composition and cellular hydration of irreversibly sickled cells.
Sickle erythrocytes (RBC) incubated under 100% nitrogen for 4 hr manifested marked Na gain with an equivalent K loss. There were no changes in cell total cation or water content under these conditions, and no irreversible sickle cells (ISC) were formed. In contrast, sickle RBC incubated for 24 hr under 100% nitrogen in a glucose-free Na medium containing calcium manifested marked ISC formation....
متن کاملDeformability of oxygenated irreversibly sickled cells.
The deformability characteristics of isolated subpopulations of irreversibly sickled cells (ISC) have been studied in an ektacytometer. Analysis of laser diffraction patterns of well-oxygenated cells subjected to shear stress in solutions of varying osmolality has demonstrated a profound influence of mean corpuscular hemoglobin concentration and intracellular viscosity on the deformability of I...
متن کاملVariable deformability of irreversibly sickled erythrocytes.
The relationship of altered deformability of irreversibly sickled cells (ISC) to their morphological and physiologic characteristics was evaluated in this study. ISC obtained by differential centrifugation and density gradient sedimentation were separated and enriched into hard and soft populations on the basis of their ability to pass through Nuclepore filters with an average pore size of 3.0 ...
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ژورنال
عنوان ژورنال: Blood
سال: 1978
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v51.6.1169.1169